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ngwaahịa

Ngwa Mgbakọ Igwe Igwe Igwe Igwe Igwe Igwe Igwe Igwe Ọdụdọ DNA Na-arụ Ọsọ nke Ọma

Nkọwa dị mkpirikpi:

CY-F006-10 (50 preps mkpụrụ ndụ)

CYF006-11 (100 preps-cell)

CY-F006-12 (200 preps-cell)

CY-F006-20 (50 nkwadebe-mmiri)

CY-F006-21 (100 preps-mmiri)

CY-F006-22 (200 preps-saliva)

Nkọwa nkwakọ ngwaahịa:

50 mmadụ / igbe, 100 mmadụ / igbe, 200 mmadụ / igbe


Nkọwa ngwaahịa

Mkpado ngwaahịa

Okwu mmalite:

Ngwa mmịpụta DNA nke Iclean na-enye usoro bead ndọta ngwa ngwa ma dịkwa mma iji sachaa & wepụ DNA (gụnyere genomic, mitochondrial na viral DNA) site na anụ ahụ echekwara, mmiri mmiri, mmiri ahụ, na buccal, cervical, sel akpụkpọ, nje nje wdg.

Enwere ike ịchekwa ihe nlere ihe gbasara ndụ na nchekwa nchekwa pụrụ iche ruo ụbọchị 30 n'ime ụlọ tupu ịhazi ya na-enweghị mfu nke mkpụrụ DNA ma ọ bụ mma (mmadụ 30 ma ọ bụrụ na echekwara ya na ọnọdụ oyi)

Enwere ike sachaa DNA genomic dị elu n'ime nkeji iri na ise na-enweghị mmịpụta phenol/chloroform ma ọ bụ mmịkọ mmanya, yana nkezi mkpụrụ DNA nke 8 μg kwa swab buccal.DNA dị ọcha, nke nwere ihe dịka 20-30 kb, dabara maka ngwa mgbada dị ka PCR ma ọ bụ mmeghachi omume enzymatic ndị ọzọ.

Uru ngwaahịa

Ịrụ ọrụ dị elu, mmịpụta DNA dị iche iche, mwepu nke protein adịghị ọcha na ogige organic ndị ọzọ na sel.Iberibe DNA ndị e wepụrụ bụ nnukwu, ịdị ọcha dị elu, kwụsiri ike na ntụkwasị obi n'ịdị mma.

1. Teknụzụ magnetik dabeere na bead iji kewapụ DNA genomic na-enweghị mkpa kemịkalụ dị ize ndụ, centrifugation, ma ọ bụ oghere manifolds, phenol na mmiri ozuzo ethanol.

2.Rapid na ịrụ ọrụ nke ọma dị ọcha nke DNA genomic site na swabs buccal mmadụ n'ime ihe na-erughị 15 nkeji na-esote nkwadebe sample na lysis.

3.Simple lysis na Proteinase K na-enweghị mkpa ọ bụla n'ibu lysis.

4.Obere mmetọ na RNA.

5.The ọcha genomic DNA egosi mma downstream arụmọrụ na ngwa gụnyere PCR.

6.Gụnyere ngwa e mere maka nhazi akpaghị aka nke ọnụ ọgụgụ dị ukwuu nke ihe nlele na 96-well plates na-eji robot njikwa mmiri mmiri.

Ojiji ngwaahịa:

Maka ịdị ọcha na ikewapụ DNA (gụnyere genomic, mitochondrial, bacterial, parasite & viral DNA) sitere na anụ ahụ, mmiri mmiri, mmiri ara, na buccal, cervical, sel akpụkpọ, cell cell, anụ ahụ, swabs, CSF, mmiri ahụ, mkpụrụ ndụ mmamịrị sachara. .

Ntuziaka ngwaahịa

1. Tupu iji nucleic acid mmịpụta reagents

①Nyefee ihe mgbaze proteinase k n'ime ntụ ntụ a mịrị amị nke nwere proteinase k wee gwakọta nke ọma.

②Tinye 18ml na 42ml nke ethanol zuru oke na CY3 na CY4 nke ụdị CY-F006-10 (50preps-cells) na CY-F006-20 (50preps-saliva), ma gwakọta nke ọma.

③.Tinye 36ml na 84ml absolute ethanol na CY3 na CY4 nke ụdị CY-F006-11 (100preps-cells) na CY-F006-21 (100preps-saliva) ma gwakọta nke ọma.

 

2. Usoro mmịpụta swab:

①Swab akọrọ mkpokọta, tinye 0.6ml CY1 mmiri mmiri, 10ul proteinase K, mix ọma, tinye ya na ikuku incubator na 65 degrees Celsius na incubate maka 30 nkeji (ma ọ bụ mmiri collection: sample centrifuge tube nke nwere swab gbakwunyere ihe nchekwa nchekwa bụ centrifuged na 12000 rpm maka nkeji 1, Debe mmiri ozuzo, wepụ ihe dị elu. Tinye 0.6ml CY1 mmiri mmiri, 10ul proteinase k, gwakọta nke ọma, tinye ya na ikuku incubator na 65 degrees Celsius na incubate maka nkeji 30).

②Wepu swab na centrifuge na 12000rpm maka 1min.

③.Wepụ ihe niile dị elu na tube centrifuge ọhụrụ wee mee nnwale ahụ.

④Tinye mmiri mmiri 0.25ml CY2, 10ul magnetik beads * (ma jijiji nke ọma tupu eji ya), gwakọta nke ọma maka 12min, tinye ya na nkwụnye magnet ka ọ kwụ ọtọ ruo 30s, ma ṅụọ mmiri mmiri.

⑤ Tinye 0.6ml nke mmiri mmiri CY3, gwakọta nke ọma maka 3min, tinye ya na nkwụnye ndọta ma hapụ ya ka ọ kwụrụ maka 30s iji ṅụọ mmiri mmiri ahụ.

⑥.Tinye 0.6ml nke mmiri mmiri CY4, gwakọta maka 3min, tinye ya na nkwụnye magnet ka ọ kwụrụ maka 30s, gbanye mmiri mmiri ahụ.

⑦.Tinyegharịa usoro ②⑥

⑧.Ghichaa maka 10-20min na ụlọ okpomọkụ, tinye 50ul CY5 mmiri mmiri maka elution, jikọta nke ọma, tinye ya na nkwụnye magnet ma hapụ ya ka ọ kwụrụ maka 30s, wee nyefee mmiri mmiri na tube centrifuge ọhụrụ.

⑨Tulee OD

 

3. Nzọụkwụ mmịpụta saliva

① Centrifuge na 12000rpm maka 1min na asu gbakwunyere ngwakọta nchekwa.

② Debe mmiri ozuzo ma wepụ ihe dị elu

③.Tinye 0.6ml nke mmiri mmiri CY1 na 10ul nke proteinase k na ya, gwakọta nke ọma, tinye ya na ikuku incubator na 65 degrees Celsius na incubate maka nkeji iri atọ.

④ Centrifuge na 12000rpm maka 1min, wepụ ihe niile dị elu na tube centrifuge ọhụrụ, tinye 10ul magnetik beads na 0.25ml CY2, gwakọta nke ọma maka 12min, tinye ya na nkwụnye magnet ma hapụ ya ka ọ kwụrụ maka 30s iji ṅụọ mmiri mmiri.

⑤ Tinye 0.6ml nke mmiri mmiri CY3, gwakọta nke ọma maka 3min, tinye ya na nkwụnye ndọta ma hapụ ya ka ọ kwụrụ maka 30s iji ṅụọ mmiri mmiri ahụ.

⑥.Tinye 0.6ml nke mmiri mmiri CY4, gwakọta maka 3min, tinye ya na nkwụnye magnet ma hapụ ya ka ọ guzoro ruo 30s iji ṅụọ mmiri mmiri ahụ.

⑦.Tinyegharịa nzọụkwụ ⑥

⑧.Ghichaa na okpomọkụ nke ụlọ maka nkeji 10-20, tinye 50ul nke mmiri mmiri CY5 maka elution, jikọta nke ọma, tinye ya na nkwụnye magnet ma hapụ ya ka ọ kwụrụ maka 30s, wee nyefee mmiri mmiri na tube centrifuge ọhụrụ.

⑨Tulee OD

Rịba ama: Ọ bụrụ na ịchọrọ iwepụ RNA, ị nwere ike ịkwadebe RNaseA 10mg / ml: ihe mgbaze (10mM sodium acetate: pH5.0), sie ya maka 15min, dozie pH 7.5 na Tris-Hcl, ma chekwaa na -20 degrees Celsius.

Ọnọdụ nchekwa na oge nkwado

1. Ekwesịrị iji ngwaahịa ahụ mee ihe na gburugburu ebe dị ọcha na ịdị ọcha, na-ezere mmetọ, ma nwee okpomọkụ kwesịrị ekwesị.

2. Na-echekwa na ụlọ okpomọkụ.Enwere ike ịchekwa Proteinase K na bead magnet na 2-8 Celsius ruo ogologo oge.

3. Ndụ nchekwa ngwaahịa: ọnwa 12

Mkpachapụ anya:

1. A na-eji ngwaahịa a naanị maka nchọpụta vitro.

2. Ebe nchekwa na usoro mmịpụta kwesịrị ịgbaso ntuziaka dị na ntuziaka.

3. Ọ bụrụ na ịchọta na ego ahụ dị ntakịrị n'oge mmịpụta, ị nwere ike ịbawanye nha nha n'ụzọ kwesịrị ekwesị ma ọ bụ mee ka ọnụ ọgụgụ nke mmịpụta dịkwuo elu.

4. DNA ewepụtara ga-adị ọhụrụ ma nwalee n'oge.

Mara: A na-eji usoro njem a maka nyocha in vitro, enweghị ike iji ya mee ihe maka ime ma ọ bụ mpụga n'ime mmadụ ma ọ bụ anụmanụ.Ọ bụrụ na e loro ya, ọ nwere ike ịkpata nnukwu ihe omume;ọ na-akpasu anya na akpụkpọ ahụ́.Ọ bụrụ na ọ na-efesa na mberede na anya, jiri mmiri sachaa ya.Ekwesịrị ịgbanye ya n'oge eji.

Okwu mmeghe nke onye nrụpụta

Huachenyang (Shenzhen) Teknụzụ Co., Ltd. ọkachamara na mmepụta nke swabs ìgwè atụrụ, akpịrị swabs, ọnụ swabs, imi imi, cervical swabs, sponge swabs, nje sample tubes, nje nchekwa ngwọta.O nwere ụfọdụ ike na ụlọ ọrụ.good

Anyị nwere ahụmịhe nrụpụta ihe karịrị afọ 12+ na ngwa ahịa ọgwụ

HCY na-ewere àgwà ngwaahịa dị ka ihe dị mkpa nke mmepe ụlọ ọrụ, na-agbaso isi ụlọ akwụkwọ "ngwaahịa mbụ, ọrụ nke mbụ" n'ụzọ niile, na-achụso mmụọ ụlọ ọrụ nke "ịchọ eziokwu, ihe ọhụrụ, ịdị n'otu na arụmọrụ" .HCY na-ahazi usoro mmepụta na ịre ahịa niile n'ụzọ siri ike dabere na usoro njikwa ISO9001 na ISO13485, yana arụmọrụ kwụsiri ike na ịdị mma a pụrụ ịdabere na ya.


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